Campy Blood-Free Selective Medium (CCDA)
Product Code: Campy Bld Free Select Med (7527)
Supplement Required: Yes
Intended Use
Campy Blood-Free Selective Medium (CCDA) is specifically designed for the selective isolation of Campylobacter species when supplemented with Cefoperazone. This medium plays a crucial role in microbiological testing, aiding laboratories in accurately identifying these pathogens.
Product Summary and Explanation
Campylobacter spp. are widely recognized as one of the leading causes of enteric illness in the United States, often resulting in diarrhea that can range from mild to severe, typically manifesting as loose, watery stools that may become bloody. These infectious agents are primarily transmitted through contaminated food or water, making effective isolation methods essential in food safety testing.
Campy Blood-Free Selective Medium (CCDA) replaces traditional blood components with a formulated blend of charcoal, ferrous sulfate, and sodium pyruvate, enhancing the growth environment for the targeted organisms. In this innovative formulation, Cefoperazone has replaced Cephazolin to further increase selectivity, reducing the possibility of non-target bacterial growth. Bolton et al. recommend incubating the inoculated plates at 37°C to significantly improve isolation efficiency.
The inclusion of amphotericin B effectively inhibits yeast and fungal contaminants, ensuring that the medium maintains its specificity for Campylobacter spp. Campy Blood-Free Selective Medium (CCDA) is particularly recommended for food testing protocols. Alongside the addition of Yeast Extract and Cefoperazone, this medium is utilized in the FDA/BAM Method for the isolation of Campylobacter species from various food samples and environmental swabs.
Key Features:
- Selective medium for isolating Campylobacter spp.
- Replaces blood with charcoal, ferrous sulfate, and sodium pyruvate for optimal growth.
- Utilizes Cefoperazone for improved selectivity over the original formulation.
- Inhibits yeast and fungal contaminants with amphotericin B.
- Recommended for use in FDA/BAM food testing methods.
