Casman Medium Base (7123)
Intended Use
Casman Medium Base is specifically designed for the isolation of fastidious microorganisms, such as Haemophilus influenzae and Neisseria gonorrhoeae, when combined with blood. Its formulation creates an optimal environment for anaerobic incubation, making it a vital choice in microbiology laboratories focused on these challenging pathogens.
Product Summary and Explanation
Originally described by Casman in 1947, this medium revolutionized the approach to culturing fastidious organisms. Unlike traditional media that relied on infusion from fresh meat and heated blood, Casman Medium Base utilizes a balanced formula enriched with 5% blood, enhancing the viability of these delicate microorganisms.
Casman was keenly aware of the biochemical interactions that could impact microbial growth. Following thorough experimentation, he identified that the presence of nicotinamide could interfere with the action of a blood enzyme responsible for inactivating V factor (NAD). In response, the concentration of nicotinamide in Casman Medium Base was carefully adjusted to promote optimal growth conditions for various Neisseria species, ensuring reliable and consistent results in laboratory applications.
Key Features and Specifications
- Medium Type: Infusion-free medium enriched with 5% blood
- Application: Isolation of Haemophilus influenzae and Neisseria gonorrhoeae
- Incubation: Anaerobic conditions to support fastidious microorganisms
- Adjustment: Optimized nicotinamide concentration for enhanced microbial growth
Optimal for Laboratory Use
The Casman Medium Base provides the precision and reliability necessary for advanced microbiological research and clinical diagnostics. Its unique formulation is essential for laboratories aiming to achieve accurate identification of fastidious pathogens.
