Intended Use
The DC Medium with BCIG is specifically designed for the chromogenic differentiation of E. coli from other coliform bacteria in water samples through the membrane filtration method.
Product Summary and Explanation
DC (Differential Coliform) Medium with BCIG provides an effective selective and differential approach for the presumptive identification of E. coli. The innovative formulation includes the chromogenic agent BCIG (5-bromo-4-chloro-3-indolyl-β-D-glucuronide), which is essential for detecting glucuronidase activity. This enzyme distinguishes E. coli from other coliforms, making it an invaluable tool in microbiological testing.
The mechanism behind the BCIG agent involves its interaction with the glucuronidase enzyme produced by most E. coli strains. Upon reacting with the enzyme, BCIG generates a color change that is visually identifiable, where the medium becomes insoluble and accumulates within the cells of the bacteria. This reaction imparts a striking blue to purple hue to the presumptive E. coli colonies, facilitating easy identification and analysis.
Key Features:
- Selective and differential medium optimized for identifying E. coli in water samples.
- Enhanced with BCIG to detect glucuronidase activity, ensuring accurate differentiation from other coliforms.
- Colorimetric change: E. coli colonies exhibit a blue to purple coloration, allowing for swift visual identification.
- Compatible with the membrane filtration method, ideal for various water sample testing applications.
DC Medium with BCIG is your precise solution for high-quality microbiological testing, providing essential results for water safety and quality assurance.
