Demi-Fraser Broth Base
Acumedia |
BD / Difco |
BD / BBL |
Oxoid |
Merck |
Demi-Fraser Broth Base (7656) |
265320 |
N/A |
CM1053 |
1.10398+ |
+ Supplement required.
Intended Use
Demi-Fraser Broth Base is specifically designed for use with ferric ammonium citrate, providing selective enrichment for Listeria species. This enhanced formulation facilitates the rapid and efficient detection of Listeria from a variety of food and environmental samples.
Product Summary and Explanation
First identified in 1926, Listeria monocytogenes poses significant risks to public health, especially for immunocompromised individuals and pregnant women. This pathogen is commonly associated with foods such as turkey frankfurters, coleslaw, pasteurized milk, Mexican-style cheese, and pâté. Listeria species are widely distributed in the environment, found in unprocessed foods, soil, sewage, and river waste.
Demi-Fraser Broth Base is a refined iteration of the original Fraser Broth Base, developed by researchers Fraser and Sperber. This broth is pivotal for the swift isolation of Listeria, adhering to AFNOR guidelines with adjusted concentrations of nalidixic acid and acriflavine. Listeria species thrive within a pH range of 5.0 to 9.6, demonstrating a notable resilience to environmental conditions that are otherwise unfavorable for many other organisms. Identification relies on the successful isolation of Listeria, followed by biochemical characterization and serological confirmation.
Utilize Demi-Fraser Broth Base in your laboratory for effective Listeria enrichment and ensure the reliability of your microbial testing protocols.