Dextrose Tryptone Broth (7338)

Intended Use

Dextrose Tryptone Broth is specifically designed for the cultivation of mesophilic or thermophilic spoilage microorganisms typically found in food. This microbiological medium is essential for detecting and isolating potential spoilage agents, ensuring food safety and quality control.

Product Summary and Explanation

Originally specified by the National Canners Association in the 1930s, Dextrose Tryptone Broth is based on the classic Dextrose Tryptone Agar formulation. It is a critical tool in microbiology for isolating "flat sour" organisms, primarily Bacillus coagulans (formerly known as Bacillus thermoacidurans), which is noted for causing spoilage in canned food products.

This unique spoilage mechanism involves a decrease in pH levels by approximately 0.3 to 0.5 without apparent swelling of the can, leading to remarkably flat can ends—a clear indicator of spoilage. B. coagulans is commonly found in soil and can contaminate canned tomatoes and dairy products, making it crucial for laboratories to monitor such organisms under favorable growth conditions to prevent food spoilage.

What sets Dextrose Tryptone Broth apart is its formulation; it features increased concentrations of Tryptone and Dextrose—doubled from its agar counterpart—while the agar component is eliminated. This allows for a more intense growth environment suitable for mesophilic and thermophilic bacteria. Recommended by authoritative entities like the American Public Health Association, this broth is particularly useful for the bacteriological examination of low and medium-acid canned foods with a pH of 4.5 and above.

Key Features

• Optimal for culturing spoilage microorganisms in food
• Enhanced concentrations of Tryptone and Dextrose for superior growth
• Validated for use in low and medium-acid canned food analysis
• Historical significance dating back to 1930s food safety practices

Specifications

Manufacturer	Catalog Number	Product Type	Application
N/A	7338	Broth	Microbiological Analysis