DNase Test Agar

DNase Test Agar (7129)

Intended Use

DNase Test Agar is specifically designed for the differentiation of microorganisms based on deoxyribonuclease activity. This specialized medium allows for the assessment of the DNase production capabilities of various bacterial and fungal species.

Product Summary and Explanation

Originally researched by Weckman and Catlin in 1956, a significant correlation was established between the DNase activity of Staphylococcus aureus and its positive coagulase activity. This groundbreaking study indicated that DNase activity could serve as a crucial identifier for potentially pathogenic staphylococci.

Building on this foundation, DiSalvo confirmed the strong relationship between coagulase and DNase activity in staphylococci derived from clinical samples. Furthermore, researchers including Jeffries, Holtman, and Guse incorporated polymerized DNA into agar to facilitate the study of DNase production in bacteria and fungi. When the medium is exposed to 1N HCl, the polymerized DNA precipitates, resulting in an opaque appearance. Microbial species capable of degrading DNA will produce a clear zone surrounding the inoculation streak, indicating their DNase activity.

In addition, studies led by Fusillo and Weiss explored the calcium requirements for DNase production in staphylococci, concluding that no additional calcium is necessary when employing a complete nutritive medium. This knowledge enhances the usability and effectiveness of DNase Test Agar in various microbiological applications.