Eosin Methylene Blue Agar, Levine

Intended Use

Eosin Methylene Blue Agar, Levine is a specialized growth medium designed for the isolation and differentiation of Gram-negative enteric bacilli. Its unique formulation allows for precise microbial analysis, making it an essential tool in clinical laboratories, food safety testing, and dairy product assessment.

Product Summary and Explanation

Eosin Methylene Blue Agar, commonly referred to as EMB, is a culture medium originally developed by Holt-Harris and Teague. This modified formula retains the essential components for effective bacterial isolation while ensuring optimal performance in identifying coliform bacteria.

The medium features lactose as the primary carbohydrate source, enhanced by two indicator dyes: Eosin Y and Methylene Blue. The Levine modification of the EMB agar involves the removal of sucrose and an increased concentration of lactose, resulting in improved detection of coliforms and other Gram-negative bacteria.

This versatile agar is suitable for testing a variety of clinical materials, as well as food and dairy products, making it a vital resource for microbiological quality control.

Technical Specifications