LB Agar, Lennox (7289)
Intended Use
LB Agar, Lennox is specifically designed for molecular genetic studies, offering a robust medium for the growth and maintenance of pure cultures of recombinant E. coli strains.
Product Summary and Explanation
Developed by Lennox, LB Agar is a nutritionally rich growth medium valued for its tailored formulation supporting the unique needs of recombinant E. coli strains, typically derived from E. coli K12. These strains are inherently deficient in B vitamin production and have been meticulously modified to create an auxotrophic strain that lacks the ability to thrive on nutritionally deficient media. LB Agar fulfills all the nutritional requirements essential for these organisms, ensuring optimal growth conditions.
In contrast to the Miller formulation of LB Agar, LB Agar, Lennox features half the sodium chloride concentration, empowering researchers to fine-tune salt levels according to the specific strain's needs. This flexibility in formulation enhances experimental accuracy and success.
Principles of the Procedure
The growth medium's nitrogen, amino acids, and carbon sources are primarily derived from the Enzymatic Digest of Casein. Essential vitamins and trace elements are supplied through Yeast Extract, ensuring a comprehensive nutrient profile. Sodium ions are incorporated via Sodium Chloride to maintain transport and osmotic balance, while Agar serves as the solidifying agent, creating a stable environment for culture growth.
Key Features:
- Nutrient-rich composition tailored for E. coli recombinant strains.
- Lower sodium chloride concentration than traditional formulations for customizable growth conditions.
- Supports the growth of auxotrophic strains, ensuring high-performance cultivation.
- Quality ingredients for reliable results in molecular genetic applications.
