LB Agar, Miller's (7213)
| Manufacturer |
Catalog Number |
Alternative Catalog Numbers |
| Acumedia |
244520 |
N/A |
Intended Use
LB Agar, Miller is specifically designed for molecular genetic studies, making it an essential medium for researchers working with recombinant strains.
Product Summary and Explanation
LB Agar, Miller’s is a nutritionally rich medium optimized for the growth of pure cultures of recombinant strains, based on the formulation outlined by Miller. This medium allows E. coli to thrive in a late log phase when grown in LB Medium. It's particularly beneficial for plasmid vectors that replicate to high copy numbers and do not necessitate selective amplification. For those vectors that have limited replication capabilities, selective amplification can be achieved by adding chloramphenicol to inhibit host synthesis, thus preventing the replication of the bacterial chromosome.
Key Features
- Contains 10 g/L of sodium chloride, differentiated from Lennox formulations, allowing for optimal salt concentration selection tailored to specific strains.
- Aseptically supplementable with glucose to enhance growth and metabolic activity.
- Ideal for researchers focusing on cloning, plasmid propagation, and other molecular biology applications.
LB Agar, Miller's is a trusted choice for scientists aiming to cultivate and study recombinant strains effectively. Its unique formulation supports versatile laboratory needs, ensuring robust performance across various genetic studies.
