LB Broth, Lennox (7290)

Intended Use:
LB Broth, Lennox is specifically designed for molecular genetic studies, crucial for the growth and maintenance of E. coli strains in research environments.

Product Summary and Explanation

Developed based on the L Broth formula detailed by Lennox, LB Broth is rich in nutrients, making it ideal for isolating pure recombinant strains of E. coli. This broth allows E. coli to thrive into the late log phase, which is essential for various molecular microbiology procedures. Some plasmid vectors can replicate to high copy numbers in LB Broth without requiring selective amplification, while others may necessitate this amplification to achieve high copy numbers.

To prevent the replication of the bacterial chromosome, chloramphenicol may be added to inhibit host synthesis, offering researchers additional control over their experiments.

Composition and Unique Features:

• Sodium Chloride Content: Contains ten times the sodium chloride level of Luria Broth, Miller, and half that of conventional LB Broth, Miller, allowing researchers to select the optimal salt concentration for their specific E. coli strain.
• Customizable Medium: Aseptically supplement the medium with glucose to prepare a complete medium as described by Lennox, tailoring it to fit particular experimental needs.

Specifications: