LB Broth, Miller's (7279)

Intended Use

LB Broth, Miller's is specifically formulated for molecular genetic studies, catering to the intricate needs of researchers and microbiologists.

Product Summary and Explanation

Based on the classic formula described by Miller, LB Broth, Miller's is an essential growth medium designed to support the cultivation and maintenance of Escherichia coli strains, which are crucial in various molecular microbiology procedures. This nutritionally rich broth facilitates the growth of pure cultures of recombinant strains, making it an indispensable component in many laboratory settings.

Key Features:

• Growth Support: Optimized for the late log phase of E. coli, enabling robust growth conditions.
• Centrally Useful: Ideal for cloning and plasmid vector work, including those requiring selective amplification.
• Highly Compatible: The formulation allows for potential supplementation with glucose, enhancing microbial life and productivity.
• Precise Composition: Contains 10 g/L of sodium chloride to maintain osmotic balance and promote cell health.

Specifications:

Applications:

LB Broth, Miller's is particularly suited for applications involving recombinant DNA technology and plasmid propagation, allowing seamless integration with various plasmid vectors. Researchers can incorporate chloramphenicol as needed to inhibit host synthesis, thereby preventing unwanted replication of the bacterial chromosome, ensuring targeted outcomes in genetic studies.