MacConkey Agar w/o Crystal Violet & Salt

Intended Use

MacConkey Agar without Crystal Violet and Salt is specifically designed for the isolation and differentiation of Gram-negative enteric bacilli. This product is especially effective for specimens that contain swarming strains of Proteus spp., ensuring accurate laboratory results.

Product Summary and Explanation

The formulation of MacConkey Agar is inspired by the bile salt-neutral red-lactose agar, originally developed to distinguish Salmonella typhosa from coliform bacteria. Over the years, enhancements have been made to promote the growth of strains such as Shigella and Salmonella. Key modifications include the addition of 0.5% sodium chloride, adjustments to agar content, and changes to bile salt and neutral red concentrations, all of which significantly improve differential reactions between enteric pathogens and coliforms.

MacConkey Agar w/o Crystal Violet & Salt serves as a differential medium that effectively inhibits the swarming behavior of Proteus spp., thereby facilitating the detection and isolation of various enteric microorganisms. By excluding sodium chloride, it creates an electrolyte-deficient environment that prevents the spreading of Proteus spp.. Additionally, the absence of crystal violet permits the growth of Staphylococcus, Enterococcus, and Mycobacterium spp., expanding the scope of microbial analysis.

Key Features:

• Isolation and differentiation of Gram-negative enteric bacilli
• Effective against swarming strains of Proteus spp.
• Excludes sodium chloride for an electrolyte-deficient medium
• Fosters the growth of Staphylococcus, Enterococcus, and Mycobacterium spp.

Specifications: