PPLO Broth w/o CV (7485)
| Brand |
Acumedia |
| BD / Difco Code |
255420 |
| BD / BBL Code |
211458 |
| Oxoid Code |
CM0403* |
| Merck Code |
N/A |
* Similar formula, but not exact.
Intended Use
PPLO Broth w/o CV is specifically designed for the isolation and cultivation of Mycoplasma species, making it an essential tool in microbial diagnostics.
Product Summary and Explanation
This broth is formulated following the methodology described by Morton and Lecce, with the deliberate exclusion of Crystal Violet. This removal is crucial as Crystal Violet has been shown to inhibit the growth of Mycoplasma, facilitating a more effective cultivation environment.
Mycoplasmas, originally identified from a case of pleuropneumonia in cattle, are classified under the Mollicutes class, renowned as the smallest free-living organisms known to science. Their size ranges between 0.2 to 0.3 micromillimeters, showcasing their unique pleomorphic characteristics.
Among the Mycoplasma species, Mycoplasma pneumoniae is notorious for causing mild forms of pneumonia, predominantly affecting individuals under the age of 40. Research indicates that Mycoplasma pneumoniae is responsible for 15% to 50% of pneumonia cases in adults, with even higher prevalence in children of school age. Common symptoms associated with this infection include headache, fever, cough, chest pain, and sore throat, necessitating the need for efficient isolation methods such as the use of PPLO Broth w/o CV.
