Terrific Broth, Modified (7708)

Intended Use

Terrific Broth, Modified is specifically formulated for use with glycerol in the cultivation of recombinant strains of Escherichia coli. This nutrient-rich broth provides an optimal environment for enhancing plasmid DNA yields.

Product Summary and Explanation

Designed by researchers Tartoff and Hobbs, Terrific Broth, Modified boasts an advanced nutrient composition aimed at significantly boosting plasmid yields from transformed E. coli. This media is widely regarded as an excellent choice for plasmid preparations, offering substantial improvements in production.

Although cells cultivated in Terrific Broth, Modified may exhibit slower growth rates compared to other liquid media, they achieve remarkable final cell densities and plasmid yields that are typically 2 to 4 times higher. This enhanced efficacy is primarily due to the optimized concentrations of Enzymatic Digest of Casein and Yeast Extract included in the formulation.

In this broth, glycerol serves as a primary carbohydrate source, presenting a distinct advantage over glucose, as it is not fermented into acetic acid. This characteristic further allows the cells to maintain an ideal growth environment, supporting robust plasmid production.

Specifications