Tetrathionate Broth Base (7241)
Intended Use
Tetrathionate Broth Base is expertly formulated for use with iodine to facilitate the recovery of Salmonella spp., providing a selective environment for their cultivation.
Product Summary and Explanation
Tetrathionate Broth Base serves as an essential selective enrichment medium specifically designed to support the growth of Salmonella spp. that may exist in low populations amidst competing intestinal flora. Certain food-processing practices can compromise Salmonella, including exposure to low temperatures, inadequate heat treatments, dehydration, radiation, and contact with preservatives or sanitizers. The presence of Salmonella spp. can lead to a range of infections, from mild gastrointestinal distress to severe conditions such as typhoid fever.
This broth was initially validated by Mueller for its effectiveness in enriching typhoid and paratyphoid bacilli while effectively inhibiting coliform organisms. Further enhancements to Mueller's original formulation by Kauffmann increased the recovery rates of positive isolates. Tetrathionate Broth has been utilized extensively within the poultry industry and has proved pivotal in collaborative studies aimed at the rapid screening of Salmonella in various food products.
Tetrathionate Broth Base, often referred to as TT Broth Base, is articulated in standard methods for the testing of Salmonella. It is incorporated within the FDA’s Bacteriological Analytical Manual as a recommended pre-enrichment medium for the detection of Salmonella in diverse food materials. Additionally, TT Broth Base is effective in processing fecal cultures for bacterial analyses.
Key Features & Specifications
- Acumedia Reference: 210430
