Tryptic Soy Broth

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Manufactured by: Neogen Inc.

Category: Clinical Culture Media

Neogen Inc. Tryptic Soy Broth

Tryptic Soy Broth (TSB) - 7164

Manufacturer Catalog Number
Multiple Sources 7164

Intended Use

Tryptic Soy Broth is tailored for the cultivation of a wide range of microorganisms. This medium is fully compliant with Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EP), and Japanese Pharmacopoeia (JP) requirements, ensuring reliable and standardized results in microbiological applications.

Product Summary and Explanation

Tryptic Soy Broth, also known as Soybean-Casein Digest Medium (abbreviated as TSB), serves as a versatile general-purpose growth medium for microbiological study. Initially developed to assess the effectiveness of sulfonamides against pneumococci and other organisms without the use of blood, TSB provides an optimal environment for various microorganisms.

This broth supports the luxuriant growth of Clostridia and non-sporulating anaerobes under anaerobic conditions and is highly recommended for testing bacterial contaminants in cosmetics, complying with stringent standards in the food industry. Additionally, it is endorsed by the USDA Animal and Plant Health Inspection Service for the detection of viable bacteria in live vaccines, making it a critical tool in both clinical and research laboratories.

The National Committee for Clinical Laboratory Standards (NCCLS) advocates the use of Tryptic Soy Broth for inoculum preparation in disk diffusion sensitivity tests, highlighting its emphasis on broad applicability. The rich nutritional base of TSB, when supplemented with sodium polyanetholesulfonate (SPS) and carbon dioxide (CO2), provides an excellent medium for blood cultures in clinical settings. Furthermore, when enriched with 6.5% NaCl, TSB facilitates the selective growth of group D streptococci, showcasing its flexibility for various testing requirements.

Key Features:

    • Conforms to Harmonized USP/EP/JP Requirements
    • Supports growth of a wide variety of microorganisms
    • Recommended for use in clinical blood culture applications
    • Endorsed by NCCLS for inoculum preparation in sensitivity tests
    • Capable of selective growth with the addition of NaCl

With its wide-ranging applications and rich formulation, Tryptic Soy Broth is an essential product for any laboratory focused on microbiological testing and research.

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