Tryptose Broth
| Product Name: |
Tryptose Broth (7367) |
| Product Code: |
262200 |
| Other Codes: |
N/A |
Intended Use
Tryptose Broth is specifically designed for the cultivation of Brucella spp. and other fastidious microorganisms. Its composition makes it suitable for various microbiological applications, providing a reliable environment for microbial growth.
Product Summary and Explanation
Tryptose Broth is uniquely prepared using Tryptose, making it highly recommended for both the cultivation and isolation of pathogenic and saprophytic bacteria. This medium emerged from studies addressing the growth requirements of Brucella spp., with Huddleson demonstrating that Tryptose media often surpassed traditional meat infusion media in effectiveness. This innovation allows for enhanced uniformity in the cultivation and differentiation of fastidious organisms.
This broth can be utilized as a complete basal medium, with the option for supplementation with enrichment agents. Notably, Huddleson documented the successful isolation of Brucella spp. from clinical specimens using a broth that contained 2% Tryptose. Moreover, McCullough et al. reported that integrating thiamine, dextrose, and iron salts significantly promotes the growth of Brucella suis.
For further enhancement, the incorporation of 0.1% agar into Tryptose Broth has been noted to improve growth rates for both aerobes and anaerobes within liquid media. The versatility of Tryptose media has solidified its recommendation in standard methods for food testing.
Key Features
- Ideal for the growth of fastidious microorganisms, especially Brucella spp.
- Can be used as a basal medium or supplemented for enhanced results.
- Supports the growth of pathogenic and saprophytic bacteria.
- Compatible with additional nutrients to optimize organism growth.
