Waters 2475 Multi-Wavelength Fluorescence Detector
The Waters 2475 Multi-Wavelength Fluorescence Detector is an advanced multi-channel, tunable fluorescence detector specifically designed for high-performance liquid chromatography (HPLC) applications. Offering outstanding sensitivity and selectivity, this detector excels at monitoring minute concentrations of target compounds and low-level impurities with exceptional precision. Whether you utilize it as a stand-alone unit or as part of an integrated chromatography system, the Waters 2475 guarantees unparalleled performance.
This state-of-the-art detector operates within an extensive wavelength range of 200 to 900 nm, incorporating newly designed optics and an enhanced illumination system which significantly boosts performance. These innovative design features enhance optical throughput and overall sensitivity, ultimately improving the signal-to-noise ratio for reliable analytical results.
Key Features:
- Innovative Axially Illuminated Flow Cell: Designed to minimize dispersion, stray light, and volume, while maximizing path length to achieve the highest sensitivity.
- Three-Dimensional Spectral Scanning: Facilitates faster method development and optimization, along with improved peak identification.
- Versatile Operating Modes: Capable of single or multi-channel operation, enabling fluorescence monitoring at one or more discrete wavelength pairs.
- Low Noise Performance: Orthogonal optics combined with mirror technology reduce stray light and minimize scatter for higher sensitivity.
- Multiple Detection Modes: Features 2D, 3D, and on-the-fly spectral scanning options, streamlining fluorescence detection method development and wavelength maxima determination.
- Integral Erbium Calibration Reference: Ensures precise wavelength accuracy for dependable analysis.
- Normalized Emission Units: Enhances bench-to-bench reproducibility, delivering consistent results across different setups.
Specifications:
| Attribute |
Specification |
| Wavelength Range |
Excitation: 200 to 890 nm
Emission: 210 to 900 nm |
| Bandwidth |
20 nm (maximum) |
| Wavelength Accuracy |
±3 nm |
| Wavelength Repeatability |
±0.25 nm |
| Sensitivity (Single Channel) |
Excitation: 350 nm
Emission: 397 nm
(Single-to-noise ratio of water Raman peak > 1000, Hamming filter TC = 1.5 sec) |
| Sensitivity Setting Range |
1 to 100,000 EUFS |
| Filter Setting Range |
Side-channel:
0.1 to 5.0 seconds (Hamming 0.1 to 99 seconds (RC)
Multichannel:
1 to 50 seconds (Hamming 1 to 99 seconds (RC) |
