Yeast Extract - Essential Microbiological Culture Media Component

Intended Use

Yeast Extract is an autolysate of yeast cells specifically used in preparing microbiological culture media. Its application is crucial in various laboratory processes and research settings.

Product Summary and Explanation

Yeast Extract is the water-soluble fraction of autolyzed yeast, carefully processed to retain naturally occurring B-complex vitamins. This product is meticulously prepared and standardized for effective use in bacteriological applications and cell cultures, acting as a powerful stimulant for bacterial growth.

Key Features:

• Concentration: Typically employed in concentrations ranging from 0.3% to 0.5% for optimal results.
• Source: Derived from the cultivation of baker's yeast, Saccharomyces spp., in carbohydrate-rich plant media.
• Preparation Method: The yeast undergoes meticulous harvesting, washing, and resuspension in water, leading to a controlled autolytic process. This self-digestion employs the yeast’s own enzymes to create a rich nutrient source.
• Finishing Process: Autolytic activity is halted by applying heat, and the resulting Yeast Extract is subsequently filtered to a clear liquid before being dried into a fine powder through spray drying.

Applications

Yeast Extract is highly regarded in culturing bacteria, particularly in studies involving milk and various dairy products. It is also extensively used in several media formulations recommended for cell culture applications, highlighting its versatility and importance in microbiological research.

Specifications