YM Broth (7363) - Ideal for Yeast Cultivation
| Brand |
Catalog Number |
Availability |
Formulation |
Applications |
| Acumedia |
271120 |
N/A |
Wickerham Formulation |
Yeast Cultivation |
Intended Use
YM Broth is specifically designed for the effective cultivation of fungi, particularly yeasts. Its targeted formulation makes it an essential medium for laboratories focusing on yeast isolation and growth.
Product Summary and Explanation
YM Broth is prepared following the well-regarded methodology published by Wickerham, who recommended its use as an enrichment medium for yeasts in environments populated by bacteria and molds. The broth's pH can be adjusted to a range of 3.0 to 4.0, enhancing its selectivity and creating optimal conditions for yeast growth.
To isolate fermentative species within your yeasts, a layer of sterile paraffin oil approximately 1 cm deep can be added to the surface of the inoculated broth. Cultures should be incubated until the appearance of growth, after which streaking onto YM Agar can help achieve isolated yeast colonies.
For comprehensive isolation of both fermentative and oxidative strains, it is advisable to place acidified inoculated YM Broth on a rotary shaker for a duration of 1 to 2 days. This method promotes effective yeast recovery while preventing the sporulation of molds, thus ensuring a successful culture process.
Moreover, the selectivity of YM Broth can be further enhanced through acidification or the incorporation of selective agents. Acidification of the broth prior to sterilization can provide additional benefits, allowing for a more effective laboratory setting for yeast cultivation.
