Brain-Heart Infusion Broth (7116)
Intended Use:
Brain-Heart Infusion Broth is expertly formulated for the cultivation of a diverse range of fastidious organisms, making it an essential component for microbiological studies and clinical diagnostics.
Product Summary and Explanation:
Initially developed by Rosenow to culture streptococci, Brain-Heart Infusion Broth has evolved through modifications by Hayden to enhance its suitability for dental pathogens. The contemporary formulation utilizes dehydrated infusions derived from porcine brain and heart tissue, creating a nutrient-rich environment conducive to microbial growth.
Enhanced by potential supplementation options, Brain-Heart Infusion Broth allows for the addition of antibiotics, varying concentrations of sodium chloride, yeast extract, and serum. These adaptations yield a robust medium suitable not only for bacteria but also for yeasts and pathogenic fungi. Furthermore, by incorporating 0.1% agar, the broth enables the reduction of oxygen tension, facilitating the growth of aerobic, microaerophilic, and obligate anaerobic microorganisms.
Brain-Heart Infusion Broth, often abbreviated as BHI, is recognized in various standards for food and water testing. It is cited by the NCCLS (National Committee for Clinical Laboratory Standards) as the recommended medium for preparing inocula in antimicrobial susceptibility testing, underscoring its importance in both research and clinical settings.
Key Features:
- Rich nutrient medium for fastidious organisms.
- Customizable formula with options for antibiotic supplementation.
- Supports growth of bacteria, yeasts, and pathogenic fungi.
- Facilitates cultivation of anaerobic and microaerophilic microorganisms.
Specifications:
- Acumedia Reference: 237500
- BD/Difco Reference: 211059
