EC Medium w/MUG

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Manufactured by: Neogen Inc.

Category: Water & Wastewater Culture Media

Neogen Inc. EC Medium w/MUG

EC Medium w/MUG (7361)

Acumedia BD / Difco BD / BBL Oxoid Merck
EC Medium w/MUG (7361) 222200 N/A CM0979 A.05101

Intended Use

EC Medium w/MUG is specifically designed for the fluorogenic detection of Escherichia coli, making it an essential tool for microbiological analysis.

Product Summary and Explanation

Originally developed by Hajna and Perry, EC Medium addresses the need for improved detection methods for the coliform group and E. coli. This unique medium features a buffered lactose broth augmented with 0.15% Bile Salts Mixture. The bile salts effectively inhibit the growth of spore-forming bacteria, while simultaneously promoting the growth of E. coli.

What sets EC Medium w/MUG apart is the incorporation of 4-methylumbelliferyl-β-D-glucuronide (MUG), which enhances detection sensitivity. This innovative approach was refined by Feng and Hartman, who introduced MUG into Lauryl Tryptose Broth at a concentration of 100 µg/mL for quick E. coli assays. Further analysis by Moburg confirmed that a reduced concentration of 50 µg/mL could still yield reliable results.

EC Medium w/MUG is prepared according to the specifications set by the US EPA, ensuring compliance with rigorous standards for water and food testing. This formulation represents a robust choice for professionals seeking reliable and effective methods for microbial analysis.

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