Rappaport-Vassiliadis R10 Broth
| Acumedia |
BD / Difco |
BD / BBL |
Oxoid |
Merck |
| Rapp-Vassiliadis R10 Broth (7512) |
218581 |
N/A |
CM0669 |
1.07700* |
* Similar formula, but not exact.
Intended Use
The Rappaport-Vassiliadis R10 Broth is specifically designed for the selective enrichment of Salmonella spp. from various food samples.
Product Summary and Explanation
Originally formulated by Rappaport and later modified by Vassiliadis, Rappaport-Vassiliadis R10 Broth offers a robust medium to enhance the isolation of Salmonella spp.. This enriched medium is distinguished from its predecessor, R 25/37°C, by its adjustment to incorporate a lower concentration of Malachite Green and a higher incubation temperature of 43°C.
Research indicates that incubation at 41.5 ± 0.5°C for 24 hours yields optimal recovery results for Salmonella spp. This broth serves as a selective enrichment medium, typically utilized after pre-enrichment of the specimen. Its utility has been validated in the analysis of various matrices, including milk and dairy products, raw flesh foods, highly contaminated foods, and animal feeds.
The selective properties of Rappaport-Vassiliadis R10 Broth are primarily due to the action of Malachite Green and the medium's intrinsic high osmotic pressure and low pH. Notably, S. typhi and S. choleraesuis demonstrate susceptibility to Malachite Green, making this broth an effective choice for ensuring the reduction of competing intestinal flora while promoting the growth of targeted Salmonella spp.
