Mueller Hinton Agar II

Intended Use

Mueller Hinton Agar is designed for antimicrobial susceptibility testing using the disk diffusion method. This formula adheres to the guidelines set forth by the Clinical and Laboratory Standard Institute (CLSI), ensuring high standards and reliability in microbiological assessments.

Product Summary and Explanation

Mueller Hinton Agar is formulated based on the original recommendations by Mueller and Hinton for the primary isolation of Neisseria species. Utilizing a simple, transparent medium, it incorporates heat-stable ingredients that ensure consistent performance. The original pea meal extract has been modified with starch, which serves as a protective colloid against potentially toxic substances, enhancing the medium’s effectiveness.

Recommended by Bauer, Kirby, Sherris, and Tuck, Mueller Hinton Agar is recognized for conducting antibiotic susceptibility tests with high-concentration disk methods. This unsupplemented medium has been endorsed by CLSI due to its low levels of sulfonamide, trimethoprim, and tetracycline inhibitors, promoting satisfactory growth for most non-fastidious pathogens while also demonstrating reproducibility across different batches.

Commonly referred to as M-H Agar, this product meets the stringent requirements set by the World Health Organization and is specified in the FDA Bacteriological Analytical Manual for use in food testing methodologies. It is effective for procedures involving both aerobic and facultatively anaerobic bacteria. Additionally, Mueller Hinton Agar can be supplemented with various additives, including 5% defibrinated sheep or horse blood, a 1% growth supplement, and 2% sodium chloride, allowing for enhanced versatility in laboratory settings.

Key Features and Specifications